As demand for the automation of biological assays has increased over recent years, the range of measurement types implemented by multiwell plate readers has broadened and the list of published software packages that caters to their analysis has …
Multiwell plate readers are an important tool in the life sciences. They are frequently used to measure fluorescence, absorbance and luminescence, among other measurement types. As they are heavily used for high-throughput assays and screens, their …
This paper presents a generalisable method for the calibration of fluorescence readings on microplate readers, in order to convert arbitrary fluorescence units into absolute units. FPCountR relies on the generation of bespoke fluorescent protein (FP) …
Contributed abstract to the 14th International Workshop on Bio-Design Automation, Paris, France, October 24th-26th 2022.
FPCount is a complete protocol for fluorescent protein calibration, consisting of 1. FP expression and production of cell lysates. 2. FP concentration determination in a microplate reader. 3. FP fluorescence quantification in a microplate reader. …
The R package FPCountR enables the calibration of microplate readers using fluorescent protein calibrants. This enables users to monitor cellular fluorescent protein expression in absolute units. More information is available on the GitHub page …