This paper presents a generalisable method for the calibration of fluorescence readings on microplate readers, in order to convert arbitrary fluorescence units into absolute units. FPCountR relies on the generation of bespoke fluorescent protein (FP) …
Contributed abstract to the 14th International Workshop on Bio-Design Automation, Paris, France, October 24th-26th 2022.
FPCount is a complete protocol for fluorescent protein calibration, consisting of 1. FP expression and production of cell lysates. 2. FP concentration determination in a microplate reader. 3. FP fluorescence quantification in a microplate reader. …
The R package FPCountR enables the calibration of microplate readers using fluorescent protein calibrants. This enables users to monitor cellular fluorescent protein expression in absolute units. More information is available on the GitHub page …